A judging panel with representatives from LBC 97.3 and Lloyds TSB Business will select the business they deem to have the best three pieces of advice and ask the company to write a weekly blog about their experiences in business, including their challenges and solutions.
During the three months, LBC 97.3 will be following the business and using their experiences to give help and advice to listeners of the show. Lloyds TSB Business experts will also join live listener discussions.
Jonathan Richards, programming director of LBC 97.3, said: "As London's biggest conversation, LBC 97.3 is a great platform for our listeners to engage in issues that really make a difference to their lives.
"This Lloyds TSB Business promotion offers not only a compelling financial reward but gives thousands of new start-ups focus, guidance and support for their own ventures."
Lloyds TSB's overall integrated campaign, which is running through to December, covers press, radio and online.
Michaela Page, campaign manager at Lloyds TSB, said: "LBC's listenership and our target audience for this campaign are closely aligned and we're excited to be doing something different."
The campaign was planned by ZenithOptimedia.