The two acts with the most votes will join heat one winners The Penny Arcade Quartet and The Salts, and the two most popular acts of heat two - double act The Lone Pepper Duo and singer Ama Ofori-Darko - to perform at the semi-final at International Confex on 19-21 March.
Below you can watch clips of all six acts – featuring singers, bands, tango dancers and a comedian. Tell us which is your favourite by scrolling down and voting in the comment box below.
The first act up is vintage-inspired singing duo Verity and Violet:
Act two is comedian Nicola Redman:
Does act three get your vote? Here's 80s tribute band Wild Boys:
Our fourth act is rock group Band Instinct:
Up next is five-piece band The Atelier, who cover a mixture of pop, jazz, rock and Latin funk:
And our sixth act is tango dancer Christian Petitto:
You have until Monday 11 March at 5pm to cast your vote in the comment box below. We’ll be tracking the voting progress on Twitter, so make sure you follow to find out which acts are in the lead.