In a statement, Amazon UK said: "We have contacted Webwise [a trading name for Phorm] requesting that we opt-out for all of our domains."
Phorm has developed technology that allows internet service providers to track what their users are doing online by scanning for keywords on websites visited. This data is sold on to media companies and advertisers that can use it to place more relevant ads on websites that the user subsequently visits.
In a statement, Phorm said: "There is a process in place to allow publishers to contact Phorm and opt out of the system, but we do not comment on individual cases."
BT trialled the UK-based firm's technology, marketed as Webwise, last year without its customer's knowledge, which provoked complaints from users and from UK members of the European Parliament.
The Open Rights Group last month wrote to the chief privacy officers at Microsoft, Google/YouTube, Facebook, AOL, Yahoo!, Amazon and Ebay urging them to opt-out of Phorm.
Amazon has so far been the only major internet firm to respond publicly.
Earlier this week the European Commission said it was starting legal action against the UK over its data protection laws in relation to Phorm's technology.
Vivian Reding, EU telecoms commissioner, said: "We have been following the Phorm case for some time and have concluded that there are problems in the way the UK has implemented parts of EU rules on the confidentiality of communications."
It is illegal in the UK to unlawfully intercept communications, but this is limited to "intentional" interception. It is considered lawful if those intercepting have "reasonable grounds for believing" that there is consent.
However, the EC's electronic privacy and personal data protection rules state that EU countries must ensure the confidentiality of communications by banning the interception and surveillance of internet users without their consent.
The UK has two months to reply to the EC's formal notification.
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