A selection of tweets will also be broadcast on the big screen before kick-off and during half-time, both inside and outside the stadium.
The featured tweets might include fans' opinions on the match, shout-outs to friends and family in the stadium, or messages of support for the finalists -- Leicester Tigers and London Irish.
Jon Duckworth, Premier Rugby's head of marketing and promotions, said: "Twitter is an instant way for us to communicate with rugby fans around the country.
"Saturday's Guinness Premiership Final is set to sell out Twickenham again for a second year running so this is a great way of interacting with the 81,601 fans in the stadium, as well bringing the atmosphere of the final to those at home."
Premier Rugby launched the Guinness Premiership Final Twitter page () to provide followers with exclusive ticket offers, updates on ticket sales and important player information.