Zucker said that Google-owned needed to do more to protect copyrighted work uploaded onto the video portal.
NBC is behind such shows as 'Law & Order', new breakout hit 'Heros', 'Scrubs', 'My Name is Earl', the US version of 'The Office' and 'E.R'.
Zucker said: "YouTube needs to prove that it will implement its filtering technology across its online platform. It's proven it can do it when it wants to.
"They have the capability. The question is whether they have the will."
The remarks are the latest in a recent spate of criticism directed at YouTube and its owner Google about the posting of clips from music videos and programmes without permission from the copyright holders.
Earlier this week, US media giant Viacom demanded YouTube immediately stop the posting of more than 100,000 video clips that belong to the company, including content from its MTV, Comedy Central and Nickelodeon channels.
Last month, News Corporation's film division 20th Century Fox issued a subpoena to YouTube, after forthcoming episodes of '24' were leaked on to the site.
Google said that when it acquired YouTube last October for $1.65bn, that it would not tolerate file sharing violations, but it has since been accused of failing to deliver on its promise.
One of the devices slated to be introduced by YouTube was a monitoring system designed to alert Google when illegal content had been posted on the site, but it has yet to materialise.
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