MySpace signed a deal with EMI's Positiva Record label, brokered by Starcom MediaVest Group, allowing it to play music video footage for the first time.
The in-stream ads will consist of five second pre- and post-roll content.
Nick Reid, UK head of sales at MySpace, said: "MySpace is synonymous with music and video, so this is a fantastic opportunity for us to directly target music enthusiasts with relevant video advertising."
The deal follows a partnership with BBC Worldwide last month to distribute clips from top BBC television programmes such as 'Doctor Who' and 'Top Gear' on MySpaceTV, which launched in June 2007.
It marked the first global agreement of this type between MySpace and a major broadcaster.