MailOnline, which includes the and sites, attracted 21.2m unique users, up 18.32% month on month.
Its UK user total increased by 11% to 6.45m users, which equates to 30% of its total audience.
held onto its top spot over Telegraph.co.uk by increasing its users by 7.4% month on month to a record of almost 26m. Its UK users total increased by a marginal 3.5% to hit 10.1m, or 39% of its total audience.
reported a small 1.15% rise in uniques to 23.2m, which was enough to hold onto second place. The site's UK users dropped by roughly 2.6% to 7m.
Following the Mail Online's impressive performance, was relegated to fourth with 20.5m unique users in October, a rise of 0.74% month on month.
However, The Times' UK users increased by about 4.6% on September to hit a record 7.1m uniques.
The Sun, which includes the as well as and , posted a 8.5% month on month increase to hit 17.1m unique users, while its UK users also rose by about 5% to 6.5m.
The also reported a rise in unique users, climbing 5% to 8.4m. The site does not release a geographical breakdown of its audience.
Mirror Group, spanning , and , reported a monthly increase in overall users, up 11% to 5.8m.
Its UK user total also jumped by almost 10% to 3.4m, which equates to 57.7% of its total audience.
The Evening Standard released figures for the for the first time, revealing a unique user figure of 1.6m for October. The site had 930,724 UK users, which was 58% of its total audience.