Organised by the London & Surrey Cycling Partnership, it is set to be the largest timed cycling event ever held in the UK, with around 20,000 cyclists expected to take part.
To mark the start of the event at the Queen Elizabeth Olympic Park, Johnson will drop a Union Flag in front of participants at 6am, before tackling the 100-mile route himself.
The Mayor will be riding on behalf of his own team, Team Boris - a 36-strong group of riders, who are aiming to raise £25,000 for his three charities.
"I am fairly confident I can get round, but how fast I can do it is another matter," said Johnson. "I’m a pretty cautious London cyclist, not a speed merchant. The chiselled whippet is yet to emerge."
The Prudential RideLondon-Surrey 100 is one of four events that make up the first Prudential Ride London festival this weekend, which is set to attract 70,000 cyclists over the two days.
The weekend will start with the Prudential RideLondon Free Cycle on Saturday morning, when 50,000 cyclists of all ages and abilities are expected to fill eight miles of traffic-free London streets.
That will be followed by the Prudential RideLondon Grand Prix, a series of sprint races around St James’s Park for professional women, handcyclists and youth riders.
On Sunday, the Prudential RideLondon-Surrey 100 will be followed by the London-Surrey Classic, a 140-mile road race between some of the top professional men’s teams in the world.
The riders will leave from Queen Elizabeth Olympic Park, before racing through the streets of east London, the City and Westminster, then out through Richmond Park and Kingston Upon Thames to the Surrey countryside, before returning for the final straight on The Mall.
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