The ad, created by TBWA\London for Adidas, shows a close-up of Wilkinson's arms in the now famous routine he goes through before taking penalty kicks.
The full-page black-and-white ad carries the line, "79th minute. All square. Impossible is Nothing", in a nod to Wilkinson's habit of kicking England to glory in the dying minutes of crucial games.
The nation is relying on the England fly-half tomorrow night in the Rugby World Cup Final against South Africa. Wilkinson is a brand ambassador for Adidas and wears the company's Predator boots.
The sportswear company said it aimed to create a tactical campaign based around the final, in an attempt to capture the mood of the nation by highlighting how Wilkinson never ceases to deliver under pressure when it counts.
The ad, called "Jonny's prayer", was devised and copywritten by TBWA\London's Danny Brooke-Taylor and Michael Burke. Media planning and buying was handled by Carat.