According to the company's blog, Spotify, which offers consumers free access to millions of music tracks online, passed the milestone at 19:50 GMT last night. Originally, Spotify operated invite only access, with open access going live on 10 February.
The Stockholm-based company, whose founders include Martin Lorentzon, previously co-founder of TradeDoubler, and Spotify chief executive Daniel Ek, opened for business in the UK, Germany, France, Italy, Spain, Finland, Norway and Sweden, on 7 October.
Since launching, the company has tied-up advertisers including the COI, Xbox, T-Mobile, Vodafone, Philips, HMV, Sainsbury's, Nissan, Sony Pictures, Paramount and Universal.
Jon Mitchell, UK sales director, said: "More innovative and curious advertisers were quick to explore how we could help them reach our rapidly growing user base. The UK press interest in Spotify over the past few weeks has definitely contributed to increased demand for our advertiser team."
Spotify's services include a free to the consumer ad-funded model, as well as premium monthly and "daily pass" ad-free subscription models, with users having on-demand access to stream millions of tracks.
The company secured licensing deals with the four majors, Universal, Sony BMG, EMI and Warner, as well as digital distributor Orchard and indie label licensing body Merlin. It recently also struck a deal with CDBaby.
Last week, the site offered users exclusive access to hear U2's new album a week before release.
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