The appeal aims to raise £50 million to improve and expand its helpline and online counselling services.
The ad is based around the strapline "Help us listen to every child's voice" and features a butterfly whose wings are made of ears.
As it flutters through a city it passes a number of children who are enjoying themselves and their fun-filled voices can be heard over a folksy sound track.
However, when it reaches a sad looking girl sat against a wall and a boy looking out of the window of a tower block there are no voices.
1st Avenue Machine and Passion Pictures created the animation in the TV ad.
The ad will be available here from 9pm tonight.
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