The posters show the rapper holding a gun in one hand and a microphone in the other. Clear Channel, the media group, last week agreed to remove posters in Los Angeles after community groups rallied against the campaign.
However, the firm has since agreed to remove 21 more posters in the Philadelphia area after they offended local anti-crime organisations.
George Kauker, president of the Philadelphia division of Clear Channel, said: "Based on clear and vocal negative response from local Philadelphia community groups, we agreed to remove this particular advertisement."
Bilal Qayyum, a leader of anti-violence group Men United for a Better Philadelphia, said: "The message could be 'rob to get rich'... It's a very offensive message that is part of a mindset that says you can solve problems with violence."
He praised Clear Channel for agreeing to remove the posters.
'Get Rich or Die Tryin'', which is due for cinema release in the US this week, stars 50 Cent as a drug dealer who spurns a life of crime to focus on rapping.
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