Dubbed 'Time to Consider', the work appeared across 35 stations on the Tube network from 20 April for two weeks, followed by a flurry of XTP activity. The campaign ends on 20 May.
The campaign featured images including that of a group of fox hunters sitting on their horses, with their backs to the camera and the copy: "Birthright or Brutality?", while an execution using a photograph of a group of battery hens carried the words "Justifiable Cruelty?"
The ads provided a web address for the Time to Consider , where consumers could comment on the ads they had seen and vote on and discuss the issues that they highlighted.
CBS conducted the campaign to prove to advertisers the potential impact of out-of-home Tube advertising.
The media owner estimated that Time to Consider prompted more than 1,000 comments on the its own website and those of organisations such as the RSPCA and League Against Cruel Sports.
Mike Hemmings, CBS Outdoor's London Underground brand manager, said: "Using a standard Underground pack, we've shown that Underground advertising can be central in leading opinion, debate and awareness, not just within London, but across the UK.
"Who knows how many conversations this has created over a pint in the pub, but the volume of online debate alone highlights how Tube ads are remembered, talked about and acted on far beyond this initial three-minute exposure."
The campaign also drew online support from organisations including Funkadelic Advertising and Support Hunting Act UK.
Hemmings said: "This campaign has shown that the unique Tube environment not only benefits advertisers with a longer dwell time in which to reach commuters, but it gives this audience the time to consider, absorb and ultimately follow up."