The 90-second ad launches on March 12 and will run for a month on satellite and cable channels including Sky, ITV3, Living TV and UKTV channels.
Most of the TV spots will air during the day in order to target women over the age of 40 who are most at risk of breast cancer.
The objective of the campaign is to help the charity differentiate itself from other breast cancer charities and generate recruitment in a crowded marketplace.
The creative, entitled "home movie", uses real footage of special moments in the lives of women with breast cancer.
All the women featured in the ad have or have had breast cancer.
Surviving relatives provide the voice-overs and the main voice-over is provided by actress Jemma Redgrave, whose mother died of breast cancer.
Permission was granted for the first time to use the music 'My Heart Will Go On', from the film 'Titanic', in an ad.
Rebecca Leuw, head of donor marketing at Breakthrough Breast Cancer, said: “We approached Watson Phillips Norman as we knew their track record on DRTV was second to none. This is a major project for us -- we are keen to extend our recruitment channels and DRTV seems an obvious medium for our cause."