Mickey and Minnie are the characters featured across the range with illustrations taken from the Disney archives being integrated into the Cath Kidston prints.
The collection is available across all categories including accessories, homeware, kids and fashion and sold in the UK, overseas and online.
Cath Kidston hopes to attract a new, younger audience after the success of its previous Disney partnership, featuring well-loved character Winnie the Pooh.
Celebrities including Fearne Cotton, Sophie Ellis-Bextor and Daisy Lowe attended the launch event on Friday.