The campaign, which contains the marketing theme "Relax", will run across portals, networks and news sites throughout August and will appear concurrently with TV and press advertising.
The agency will use its own planning tool, Brand Gauge, to demonstrate the effectiveness of the campaign. The creative was produced by Dare.
Richard Dance, head of strategy at Unique Digital, said: "It is telling that such a major player in the UK finance industry is actively testing this important opportunity. With our research partner, Dynamic Logic, we will be able to show not only the impact of exposure to online advertising on brand metrics, but also at what point frequency becomes most effective in achieving our objective."
Colin Tomkinson, internet recruitment programme manager at Barclaycard, added: "We are hoping to be able to prove that not only can the internet be a very effective customer acquisition tool, but online display advertising can contribute to a positive brand response."
Unique has a long-term relationship with Barclaycard, which is set to continue, and the digital agency is also working with Barclays Bank.
Walker Media won the £70m Barclays media planning and buying account in March this year, after assuring the bank that it would staff up its online division, Walker-i.
The account has now moved out of previous agency Starcom UK, but Unique is taking on some digital work for the bank in the short term.
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