The 60-second ad, which was created by DDB Chicago, shows a horse called Hank suffering the ignominy of being rejected to help pull the Budweiser wagon.
A horse called Thunder is chosen in his place, while Hank stands alone rejected and humiliated. As he does, a black and white spotted Dalmatian watches him from under a tree.
A Budweiser commercial has been voted the most popular ad during last night's Super Bowl, with a spot that pays homage to Sylvester Stallone's 'Rocky', but instead of a boxer the ad shows a Dalmatian training a horse.
The ad then shows the Dalmatian training Hank to the stirring sound of 'Rocky''s signature theme tune 'Gonna Fly Now'. Hank is shown weaving in and out of the trees in a dense forest in the depths of winter, lifting bales of hay and pulling a train.
Following months of rigorous training, the ad then shows Hank being chosen to pull the Budweiser wagon one year later. It ends with the strapline: "The Great American Lager".
The ad polled the highest rating in USA Today's Super Bowl Ad Meter, giving parent company Anheuser-Busch the accolade for a record 10th year in a row.
The second most popular ad in the poll was claimed by FedEx with an ad in which the company is shown competing with giant carrier pigeons. Third spot was claimed by tyre maker Bridgestone for an ad showing a car narrowly missing running over a squirrel.
Fourth spot was taken by Doritos for an ad showing a giant rat going for a man's bag of crisps; while fifth was staked by Bud Light with an ad showing a fire-breather heating up a romantic dinner.
Last year's winning ad for Budweiser featured a group of crabs worshipping a cooler of Budweiser on the beach.
Budweiser also paid for a spot to promote Bud Light, the ad pokes fun at celebrity endorsements and features Will Ferrell filming a fake commercial as a 1970s basketball player-coach named Jackie Moon. Ferrell will play the Moon character in his next movie 'Semi-Pro', which is due out at the end of the month in the US.
Other celebrity appearances included pop star Justin Timberlake in a spot for Pepsi. The campaign, created by BBDO New York and directed by Craig Gillespie, promotes Pepsi Stuff, a new marketing initiative that features the soft drinks maker giving away music and other items in return for points.
Another poll, this time by HCD Research, named the FedEx pigeon ad as the top Super Bowl commercial. The "carrier pigeons" spot was created by BBDO NY.
Audi returned to the Super Bowl for the first time in 20 years with a 'Godfather'-inspired spot created by Venables Bell & Partners. You can view the ad below.