The campaign, created and handled by Specsavers Creative, includes TV, online, outdoor, press and in-store branding activity. The stores' high-street employees will also wear branded t-shirts.
The TV ad shows Mr Happy waking up in Happyland in a bad mood. He is angry with Mr Tickle, Mr Bump, Mr Messy and Mr Greedy because he missed the latest Specsavers offer.
The ad is the latest to use the slogan "Should've gone to Specsavers" and is designed to promote six new Specsavers offers, which each correlate to a different 'Mr Men' character. Mr Greedy's offer equals a free second pair of glasses while Mr Cool's is a free sunglasses treatment.
Graham Daldry, creative director of Specsavers Creative, said: "Following the success of our 2009 'Postman Pat' campaign we expect 'Mr Men' to have a similarly massive standout.
"The tone of voice fits perfectly with our other advertising – great offers delivered with genuine humour and warmth. The best way to get information across to people is to entertain them."
The promotion runs until 1 January 2011.