The chain will place more importance on launches, seasonal promotions and books on topical subjects and local issues.
Borders is aiming to establish itself as the leading store for depth of range ahead of rivals such as Waterstones. It also plans to implement the changes across its Books Etc subsidiary.
The company is trialling a fresh layout at the front of its stores, usually seen as an ideal position to place discounted books, aimed at consumers who want to avoid searching through the main range.
It intends to create the immediate impression that each store houses a large number of titles.
Other changes will include the refreshing of all in-store signage and a display of its bestsellers.
Although discounting will continue, the offers will now be pushed further back into the store.
Borders marketing promotions manager Meg Seymour said: 'There is a challenge now from websites and the supermarkets, and we are making changes like this so that we can gain that point of difference.'
The revamped store layouts are being trialled in Swindon and Oxford, and are due to be rolled out to Borders' 30 remaining superstores and Books Etc's high-street outlets this year.
Borders UK managing director Philip Downer said the plan is to move away from 'default discount offers to a variety of promotions which suit large, small, specialist or popular titles'.