The outdoor campaign, which was devised by experiential communications agency Not Actual Size, will take place over the next few days in and around central London.
It will feature a 26ft high animatronic of Miami Dolphins' defensive end Jason Taylor, dubbed "Big JT", in London's Trafalgar Square. Created by Artem, the figure weighs more than a tonne and is dressed in the world's largest football jersey.
The "Big JT" will be taking a "mystery tour" through London in the lead up to Sunday's at Wembley Stadium. It will be accompanied by an NFL tailgate party, featuring traditional American food, Miami Dolphin cheerleaders and live music.
The areas set to receive a visit include Canary Wharf, Victoria Station and Lakeside shopping centre in Kent, among others.
The game, which sold out in two hours, marks the first time that two NFL sides have played a competitive league match outside the US. It will be broadcast live on Sky Sports 2 and BBC Radio 5, with highlights to appear on Sunday night's 'Match of The Day 2' on BBC Two.
Miami Dolphins have been given home team rights for the game, after forfeited one of their own home games in order to play the London fixture.
The London game and campaign was devised to foster an audience for American Football outside the US. It is understood that further overseas NFL games have been planned until 2011, with two matches a season slated for Germany, Canada, Mexico and the UK.
The NFL has been keen to emphasise that the game between the Miami Dolphins and the New York Giants will not be an exhibition match, but an intense midseason clash between two of the league's biggest teams.
Meanwhile, Roger Goodell, NFL commissioner, caused controversy among US sports fans earlier this week by suggesting that growing interest in American Football abroad could lead to the Super Bowl being held in London. However, Goodell later said the league had "no plans" to hold the sport's biggest event abroad.
Alastair Kirkwood, managing director of NFL UK, said: "[The match] will be the first time that a competitive NFL game is being played outside of the United States. So it seems only fitting that we create something that reflects the scale of this event, and it doesn't come much bigger than a 26ft tall American Football player."
The NFL has previously tried to launch the NFL outside of the US with NFL Europe in the 1990s, which gave the UK the London Monarchs, later the England Monarchs; however, the team was folded in 1999.