The 90-second ad, created by Mother and directed by Kim Gehrig through Academy Films, carries the strapline "You are powerful" and shows a range of ordinary people physically preventing human rights abuse.
It follows seven people, including a father, a young teenager, an elderly woman and a pregnant woman, intervening in situations of human rights violence and abuse.
The ad was first screened during Amnesty's comedy fundraising event the 2008 Secret Policeman's Ball, staged at the Royal Albert Hall on October 4.
It also aired without any Amnesty branding during the event's television broadcast on Channel 4.
Matthias Stock, brand planner at Amnesty International, said that the aim of the film was to make an impact at the ball and at future Amnesty events.
The film will launch online on December 10 and Amnesty is aiming to secure donated media space in order to roll out a branded version on TV and in cinemas.
Amnesty's ad was voted October winner of the Thinkboxes ahead of Barclaycard's "waterslide" and VW Tiguan's "Walter".
Stock said: "We wanted to inspire people to use their voice to effect change. The key insight behind the film was telling people that they can be powerful -- you just have to believe it."
To see the full shortlist go to .