The global fast food chain is to build a two-storey restaurant at the Olympic site in Stratford, East London, in time for the opening of the games next year.
McDonald's is set to announce further deals of the venture this afternoon when it also unveils details of its sponsorship of London 2012.
The 3,000-square-metre restaurant will dwarf all other of the burger-chains estimated 32,000 restaurants across the globe.
The restaurant will be one of four on the Olympic site, including one located in the athlete's village.
The fast food outlet is expected to serve thousands of Big Macs and fries during the four weeks that the Olympics and the Paralympics are held, served by more than 470 staff.
Next year's London Olympics will be the ninth consecutive games that McDonald's has acted as the event's official restaurant and the only branded food service retailer feeding the athletes.
In April, McDonald's reported global profit of $1.2bn (£730m), up 10.9% from the previous year as revenue rose 9% to $6.1bn.
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