Pudsey's fresh look was created by Fallon London. He has been modernised to work online in animated poses. As part of the makeover, Pudsey will wear a variety of multicoloured eye patches.
The charity will also unveil the strapline 'Do something different', which will be supported by a website aimed at helping raise more than £33m this year.
The BBC hopes the activity will help it sell more Pudsey-branded products, which currently account for only 3% of the charity's revenue. It will now aim to attract more licence partners to raise an incremental £1m over the next three years. Last year, almost 100,000 stuffed Pudsey bear toys were sold.
The relaunch will also be communicated through an integrated campaign, including a direct mailing pointing supporters to the website.
The mailer, which includes a fundraising pack, will be supported by TV spots created by Red Bee Media that break on BBC One next month.
As well as boosting its fundraising initiative, the BBC hopes that the revamp will dispel negative viewer perceptions of the programme, which, earlier this summer, was among several BBC charity shows revealed to have deceived viewers with rigged phone-ins.
This year's Children in Need takes place on Friday 16 November.