The e-tron room launched in Barcelona last month and welcomed 800 participants over three weeks.
Audi said the one-hour experience is "the most technological escape room in history" and it aims to help people understand electric cars.
As part of the game guests will be on a mission to find a missing doctor. The players will have to work with technology such as robotic arms, holograms, interactive projection mapping, mechanical installations to solve the clues.
The escape room will be in Madrid between 9 and 30 November.
Audi’s marketing director Caíta Montserrat said: "We think this is a fun way for the public to get to know the future of electric cars. A future which will become a reality next year with the Audi e-tron quattro, which will be equipped with cutting-edge, 100% electrically-powered technology, assuring driving distances of more than 500 kilometres."